This is a list of notable events, premieres, releases, series cancellations, endings and other business ventures for Nickelodeon in the year 2000.
- January 22 - Double Dare 2000 debuts on weekday afternoons.
- March 11 - Caitlin's Way begins its run on Sunday nights.
- July 23 - The Brothers Garcia begins its run on Sunday nights.
- August 14 - Dora the Explorer (Nick Jr.)
- October 2 - Pelswick, an Canadian cartoon, created by cartoonist John Callahan premieres, before new episodes air Tuesday and Wednesday nights.
- October 7 - Noah Knows Best
- October 25 - As Told by Ginger premieres in primetime.
- January 15 - KaBlam!, 4 seasons
- April 23 - The Journey of Allen Strange
- July 15
- August 25 - Mr. Wizard's World, one of Nickelodeon's longest-running shows, finally ends its run.
- August 30 - Allegra's Window and Gullah Gullah Island
- November 10 - Double Dare 2000 ends after 2-and-one-half seasons.
- January 1 - Nickmillenium (worldwide and Noggin) All Day
- January - Slime Time Live premieres.
- January 12 - Nick Flicks ends.
- June 28 - The SpongeBob's Nicktoon Summer Splash summer block premieres.
- The Nickel-o-Zone ends.
- July 22 - Kenan & Kel: Two Heads Are Better Than None, the series finale for Kenan & Kel, and Nickelodeon's first made-for-TV movie, premieres.
- October 28 - Cry Baby Lane
- November 25 - CatDog: The Great Parent Mystery
- November 12 - Rugrats in Paris: The Game
- Chuckie Meets the Beastie Bunny
- Open Wide: A Visit to the Dentist
- A Tale of Two CatDogs
- May - Rugrats Discover America (book)
- July 1 - It Takes Two!
- September 1
- January 11 - Rugrats: I Think I Like You
- May 5 - Rugrats: Discover America
- October 3 - Snow Day
- March 6 - Jacob Bertrand is born
- March 21 - Jace Norman is born
- April 21 - Rolante Yomti is born
- June 2 - Lilimar is born
- August 20 - Fatima Ptacek is born
- October 31 - Willow Smith is born
Nickelodeon character debuts